The Application of Response Surface Methodology in Hydrotropic Microwave Assisted Extraction of Andrographolide

Document Type : Original Article


1 Department of Chemical Engineering Wahid Hasyim University, Semarang, Indonesia

2 Department of Pharmacy Wahid Hasyim University, Semarang, Indonesia


Andrographolide of Andrographis paniculata is reported has a broad category of
pharmacological activity. Various attemps have been done in order to improve the
andrographolide extraction performance. Hydrotropic-microwave assisted extraction provides an
effective method in andrographolide extraction. In this study, Response Surface Methodology
(RSM) along with central composite design (CCD) was applied to optimize the hydrotropicmicrowave assisted extraction of andrographilde. The effect of extraction time (X
1), solid-liquid
ratio (X
2), and hydrotropes concentration (X3) were investigated. The result showed that the most
suitable condition for extraction of andrographolide was found to be extraction at 32,5 minutes,
solid liquid ratio of 0,095 and hydrotropes concentration of 4M. The maximum extraction
percentage experimentally (5,45%) was found to be very close to its predicted value. It was also
found that hydrotropes concentration was the most influencing variable. The mathematical model
for the hydrotropic-microwave assisted extraction is :
Y = 4.812 + 1.66 X
1 - 2.366 X 1
+ 0.478 X
2 - 2.473X 2
+ 2.216 X
3 - 0.983X 3
1X 2+0.476 X 1X 3+0.429 X 2X 3